My reseach is mainly on developing the drug-induced premature condensed chromosome (PCC) technique and its application in many area of cytogenetics. The drugs used here are Calyculin A, Okadaic acid or other inhibitors (Cantharidin, Tautomycin, Mycrocystin LR) of protein phosphatases.This technique allows the interphase nuclei to be visualized like as metaphase chromosomes. Therefore, it is able to analyze the nuclei as chromosome form.

Listed the below are papers which related on my work,in particular on drug induced PCC, and my papers cited in these papers.

This Graph shows the Number of published Papers that use or apply the drug-induced PCC technique, and shows the cited times (primary or secondary citation) of my published papers since I published my first drug-induced PCC phenomenon using phosphatase inhibitors, okadaic acid or calyculin A ( Gotoh et al., Biomedical Research 1995 Jan;16(1):63-68. [Gotoh 1995] ). It cleary shows how a lot of these published articles were inspired by my first report [Gotoh 1995] .

Last Updated on [an error occurred while processing this directive]

	Literature		Times Cited serched by ISI WEB of SCIENCE , Google Scholar and PubMed
	1. Ohsawa K, Yamagami T, Nishizawa M, Inoue C, Gotoh E, Yamamoto H, Okamoto H., Structure and expression of Human Vasoactive Intestinal Peptide/PHM-27 Gene. Gunma Symposia on Endocrinology. 1987;24:169-181.		5
	2. Yamagami T, Ohsawa K, Nishizawa M, Inoue C, Gotoh E, Yanaihara N, Yamamoto H, Okamoto H., Complete nucleotide sequence of human vasoactive intestinal peptide/PHM-27 gene and its inducible promoter. Ann N Y Acad Sci. 1988;527:87-102.		55
	3. Gotoh E, Yamagami T, Yamamoto H, Okamoto H., Chromosomal assignment of human VIP/PHM-27 gene to 6q26----q27 region by spot blot hybridization and in situ hybridization. Biochem Int. 1988 Sep;17(3):555-562.		16
	4. Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K., Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet. 1995 Feb;95(2):233-234.		0
	5. Eisuke Gotoh, Yohko Asakawa, Hiroshi Kosaka. Inhibition of protein serine/threonine phosphatases directly induces premature chromosome condensation in mammalian somatic cells. Biomed Res, 16, 63-68, 1995. PDF is also avairable from here		136
	6. Eisuke Gotoh, Yohko Asakawa. Detection and evaluation of chromosomal aberrations induced by high doses of gamma-irradiation using immunogold-silver painting of prematurely condensed chromosomes. Int J Radiat Biol, 70, 517-520, 1996.		68
	7. Yohko Asakawa, Eisuke Gotoh. A method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold-silver staining. Mutagenesis, 13, 175-177, 1997.		35
	8. Gotoh E, Ohishi A, Kawakami K. Ga-67 imaging in a rare case of Bellini duct carcinoma.. Clin Nucl Med, 23, 46-47, 1998.		4
	9. Gotoh E, Ohsawa T, Kato K, Kawakami K. Ga-67 imaging of thiamazole-induced agranulocytosis associated with multiple focal infections. Clin Nucl Med, 23, 114-116, 1998.		4
	10. Durante M, Furusawa Y, Gotoh E. A simple method for simultaneous interphase-metaphase chromosome analysis in biodosimetry. Int J Radiat Biol, 74, 457-462, 1998.		204
	11. Durante M, Furusawa Y, Majima H, Kawata T, Gotoh E. Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes. Radiat Res, 151, 670-676, 1999.		89
	12. Gotoh E, Kawata T, Durante M. Chromatid break rejoining and exchange aberration formation following gamma-ray exposure: analysis in G2 human fibroblasts by chemically induced premature chromosome condensation. Int J Radiat Biol, 75, 1129-1135, 1999.		78
	13. Johnson RT, Gotoh E, Mullinger AM, Ryan AJ, Shiloh Y, Ziv Y, Squires S. Targeting double-strand breaks to replicating DNA identifies a subpathway of DSB repair that is defective in ataxia-telangiectasia cells. Biochem Biophys Res Commun, 261, 317-325, 1999.		60
	14. Kawata T, Gotoh E, Durante M, Wu H, George K, Furusawa Y, Cucinotta FA. High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts.Int J Radiat Biol, 76, 929-937, 2000.		63
	15. Kawata T, Durante M, George K, Furusawa Y, Gotoh E, Takai N, Wu H, Cucinotta FA. Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations. Phys Med, 17 suppl 1, 226-228, 2001		3
	16. Ito S, Gotoh E, Ozawa S, Yanagi K. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular. J Gen Virol, 83, 2377-2383, 2002		43
	17. Takakura K, Gotoh E, Sakano A, Funada A, Kanasugi Y, Okabe A, Kobayashi K., Chromosomal aberrations in normal human cells induced by the auger effect via Ca atoms., Int J Radiat Biol. 2004;80(11-12):881-888.		5
	18. Gotoh E, Tanno Y, Takakura K.., Simple biodosimetry method for use in cases of high-dose radiation exposure that scores the chromosome number of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC)., Int J Radiat Biol. 2005 Jan;81(1):33-40.		68
	19. Mochida A, Gotoh E, Senpuku H, Harada S, Kitamura R, Takahashi T, Yanagi K., Telomere size and telomerase activity in Epstein-Barr virus (EBV)-positive and EBV-negative Burkitt' lymphoma cell lines., Arch Virol. 2005 Oct;150(10):2139-2150. Epub 2005 Jun 28.		23
	20. Gotoh E, Tanno Y., Simple biodosimetry method for cases of high-dose radiation exposure using the ratio of the longest/shortest length of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC).,Int J Radiat Biol. 2005 May;81(5):379-385.		44
	21. Gotoh E, Durante M., Chromosome condensation outside of mitosis: Mechanisms and new tools., J Cell Physiol. 2006 Nov;209(2):297-304 and Front Cover Page		96
	22. Tanno Y, Kobayashi K, Tatsuka M, Gotoh E, Takakura K.,Mitotic arrest caused by an X-ray microbeam in a single cell expressing EGFP-Aurora Kinase B., Radiat Prot Dosimetry. 2006 122(1-4):301-306. Dec 13; [Epub ahead of print]		12
	23. Gotoh, E., Visualizing the dynamics of chromosome structure formation coupled with DNA replication., Chromosoma. 2007 Oct;116(5):453-462. Epub 2007 May 15.		35
	24. Gotoh, E., Funada, A., Mohri, M, Lee, R., Takakura, K., High yields of isochromatid breaks and successive formation of chromosome exchanges may lead to reproductive cell death following high-LET irradiation.,Central European Journal of Biology. 2008 3(2):121-133. Full Text (PDF) is here.		1
	25. Ogi, S., Gotoh, E., Uchiyama, M., Fukuda, K., Urashima, M., Fukumitsu, N., Influence of hilar deposition in the evaluation of the alveolar epithelial permeability on 99mTc-DTPA aerosol inhaled scintigraphy., Jpn J Radiol, 2009, 27(1), p. 20-24.		12
	26. Gotoh, E.,Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin", In: S.P. Chellappan (Eds.): Chromatin Protocols: Second Edition, Vol. 523, Humana Press, 2009, pp. 83-92. (Publisher) or (PubMed)		22
	27. Gotoh E.,Visualize Dynamic Chromosome., Atlas of Genetics and Cytogenetics in Oncology and Haematology (Online publishing Journal), January 2011. PDF version is available from here		2
	28. Gotoh E.,Visualize Dynamics of Chromosome Structure Formation and DNA Repair/Recombination Coupled With DNA Replication: Tight Coupled Role of DNA Replication in Chromosome Compaction and DNA Recombination, Chapter 8 Fundamental Aspects of DNA Replication, p 119-144, Jelena Kušic-Tišma (Ed.), ISBN: 978-953-307-259-3, InTech, September 2011. PDF version is available from here:		1
	29. Gotoh E., Cytogenetic biodosimetry for accidental emergency irradiation exposure preparedness, in particular merit of the use of drug-induced premature chromosome condensation (PCC) with calyculin A, , Atlas of Genetics and Cytogenetics in Oncology and Haematology (Online publishing Journal), July 2012. PDF version is available from here		3
	30. Gotoh E., Premature Chromosome Condensation (PCC): Tools in chromosome and cytogenetic research., Atlas of Genetics and Cytogenetics in Oncology and Haematology (Online publishing Journal), May, 2013 PDF version is available from here		0
	31. Gotoh E., Drug-induced premature chromosome condensation (PCC) protocols: cytogenetic approaches in mitotic chromosome and interphase chromatin.		9
	32 Gotoh E.,G2 premature chromosome condensation/chromosome aberration assay: Drug-induced premature chromosome condensation (PCC) protocols and cytogenetic approaches in mitotic chromosome and interphase chromatin for radiation biology		2
			Total 1216

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